DETERMINATION OF CYANOGENIC GLUCOSIDES IN WHITE CLOVER (TRIFOLIUM-REPENS L) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:11
作者
STOCHMAL, A [1 ]
OLESZEK, W [1 ]
机构
[1] INST SOIL SCI & PLANT CULTIVAT,DEPT BIOCHEM,PL-24100 PULAWY,POLAND
关键词
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LINAMARIN; LOTAUSTRALIN; QUANTITATIVE ANALYSIS;
D O I
10.1002/pca.2800050511
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high performance liquid chromatographic method used for the determination of cyanogenic glucosides in flax seed has been modified to make it suitable for the analysis of these compounds in dried white clover (Trifolium repens L.) samples. Moreover, the extraction of glycosides from clover samples and purification of extracts on the C18 support prior to analysis has been developed. Linamarin and lotaustralin were identified in T. repens cv. Armena in concentrations of 1.29 and 3.13 mg/g of dry matter, respectively.
引用
收藏
页码:271 / 272
页数:2
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