FLUOROMETRIC ASSAY OF ENZYMATIC REACTIONS INVOLVING ACETYL COENZYME A IN ALDOL CONDENSATIONS

被引：31

作者：

CALVO, JM

BARTHOLO.JC

STIEGLIT.BI

机构：

[1] Department of Biochemistry and Molecular Biology, Cornell University, Ithaca

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(69)90168-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis and properties (NMR, UV absorption, fluorescence emission spectra, and fluorescence quantum yield) of umbelliferone, 4-isopropylumbelliferone, and 4-carboxymethylumbelliferone are described. The optimal conditions for the formation of these umbelliferones from substituted malic acids and resorcinol have been determined. This forms the basis for a rapid and sensitive fluoremetric assay of enzymes catalyzing aldol condensation reactions involving acetyl coenzyme A. © 1969.

引用

页码：164 / &

共 31 条

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[5] ABSOLUTE CONFIGURATION OF ALPHA-HYDROXY-BETA-CARBOXYISOCAPROIC ACID ( 3-ISOPROPYLMALIC ACID ) AN INTERMEDIATE IN LEUCINE BIOSYNTHESIS
CALVO, JM
STEVENS, CM
KALYANPUR, MG
UMBARGER, HE
[J]. BIOCHEMISTRY, 1964, 3 (12) : 2024 - +
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