INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 INHIBITS BINDING OF IGF-I ON FETAL SKIN FIBROBLASTS BUT STIMULATES THEIR DNA-SYNTHESIS

被引:52
作者
KOISTINEN, R
ITKONEN, O
SELENIUS, P
SEPPALA, M
机构
[1] Department of Obstetrics and Gynecology, Helsinki University Central Hospital, SF-00290 Helsinki
基金
芬兰科学院;
关键词
D O I
10.1016/S0006-291X(05)81073-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Insulin-like growth factor-binding protein-1 (IGFBP-1) was purified from human midtrimester amniotic fluid using monoclonal anti-IGFBP-1 affinity column. Two peaks were obtained in anion exchange chromatography. Both had the same molecular mass of 30 kDa. In monolayer cultures of fetal skin fibroblasts both forms of IGFBP-1 inhibited binding of [125I]IGF-I onto the cells, but amplified the IGF-I-stimulated [3H]thymidine incorporation into the same cells. Radiolabeled IGFBP-1 did not bind to the cells. No detectable IGFBP-1 was released into conditioned medium from the cells, and they contained no specific IGFBP-1 mRNA. Recently we found that the same IGFBP-1 preparation inhibits IGF-I-stimulated [3H]thymidine incorporation into human hyperstimulated granulosa cells (1). These results show that, depending on target cells, the same protein is capable of either stimulating or inhibiting DNA synthesis. © 1990 Academic Press, Inc.
引用
收藏
页码:408 / 415
页数:8
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