ESR SPIN TRAPPING DETECTION OF HYDROXYL RADICALS IN THE REACTIONS OF CR(V) COMPLEXES WITH HYDROGEN-PEROXIDE

被引：36

作者：

SHI, XL ^{[1
]}

DALAL, NS ^{[1
]}

机构：

[1] W VIRGINIA UNIV, DEPT CHEM, MORGANTOWN, WV 26506 USA

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 10卷 / 1-2期

关键词：

Cr(V); Cr(VI) reduction; ESR; Free radicals; Hydrogen peroxide; Spin trapping;

D O I：

10.3109/10715769009145929

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：17 / 26

页数：10

共 40 条

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