THE 3RD SUBUNIT OF DESULFOVIRIDIN-TYPE DISSIMILATORY SULFITE REDUCTASES

被引：51

作者：

PIERIK, AJ ^{[1
]}

DUYVIS, MG ^{[1
]}

VANHELVOORT, JMLM ^{[1
]}

WOLBERT, BG ^{[1
]}

HAGEN, WR ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,BIOCHEM LAB,DREIJENLAAN 3,6703 HA WAGENINGEN,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 205卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb16757.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In addition to the 50-kDa (alpha) and 40-kDa (beta) subunits, an 11-kDa polypeptide has been discovered in highly purified Desulfovibrio vulgaris (Hildenborough) dissimilatory sulfite reductase. This is in contrast with the hitherto generally accepted alpha(2)beta(2) tetrameric subunit composition. Purification, high-ionic-strength gel-filtration, native electrophoresis and isoelectric focussing do not result in dissociation of the 11-kDa polypeptide from the complex. Densitometric scanning of SDS gels and denaturing gel-filtration indicate a stoichiometric occurrence. A similar 11-kDa polypeptide is present in the desulfoviridin of D. vulgaris oxamicus (Monticello), D. gigas and D. desulfuricans ATCC 27774. We attribute an alpha(2)beta(2)gamma(2) subunit structure to desulfoviridin-type sulfite reductases. N-terminal sequences of the alpha, beta and gamma-subunits are reported.

引用

页码：111 / 115

页数：5

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