FATTY-ACID HYDROPEROXIDE LYASE IS A HEME PROTEIN

被引:49
作者
SHIBATA, Y [1 ]
MATSUI, K [1 ]
KAJIWARA, T [1 ]
HATANAKA, A [1 ]
机构
[1] UNIV E ASIA,GRAD SCH INTEGRATED ARTS & SCI,DEPT LIFE SCI,SHIMONOSEKI,YAMAGUCHI 751,JAPAN
关键词
D O I
10.1006/bbrc.1995.1207
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fatty acid hydroperoxide lyase (HPO lyase) is an enzyme that cleaves hydroperoxides of polyunsaturated fatty acids to form short chain aldehydes and omega-oxoacids. Spectrophotometric analyses of HPO lyase highly purified from green bell pepper fruits indicate that it is a heme protein. The heme species was revealed to be heme b (protoheme IX) from the absorption spectrum of the pyridine hemochromogen. Although the spectrum highly resembles that of a plant cytochrome P450, allene oxide synthase from flaxseed, CO treatment of the enzyme caused no appearance of a peak at 450 nm, which is an essential diagnostic feature of a cytochrome P450. Internal amino acid sequences determined with peptide fragments obtained from the lyase showed no homology with any reported sequences. (C) 1995 Academic Press, Inc.
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页码:438 / 443
页数:6
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