CLONAL DETERMINATION OF UTERINE LEIOMYOMAS BY ANALYZING DIFFERENTIAL INACTIVATION OF THE X-CHROMOSOME-LINKED PHOSPHOGLYCEROKINASE GENE

被引：77

作者：

HASHIMOTO, K ^{[1
]}

AZUMA, C ^{[1
]}

KAMURA, T ^{[1
]}

NOBUNAGA, T ^{[1
]}

KANAI, T ^{[1
]}

SAWADA, M ^{[1
]}

NOGUCHI, S ^{[1
]}

SAJI, F ^{[1
]}

机构：

[1] CTR ADULT DIS,DEPT SURG,OSAKA 537,JAPAN

来源：

GYNECOLOGIC AND OBSTETRIC INVESTIGATION | 1995年 / 40卷 / 03期

关键词：

UTERINE LEIOMYOMA; X-INACTIVATION; DIFFERENTIAL METHYLATION; CLONALITY; POLYMORPHISM; PHOSPHOGLYCEROKINASE;

D O I：

10.1159/000292336

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

To investigate the clonality of uterine leiomyomas, we developed a PCR-based method involving the differential inactivation of the X-chromosome linked phosphoglycerokinase (PGK) gene. Small DNA samples of 22 leiomyomas from 9 Japanese patients, showing heterozygosity at the BstXI site of the PGK gene, were digested with the methylation-sensitive restriction enzyme HpaII. Only the inactive (methylated) PGK gene allele was selectively amplified by PCR followed by digestion with BstXI and electrophoresis. All leiomyoma samples consisted of a single type of inactive allele, even though alleles were detected that were specific to each nodule. The results indicated that all leiomyoma nodules were unicellular in origin but independently generated in the uterus.

引用

页码：204 / 208

页数：5

共 22 条

[1] EXPRESSION OF THE INSULIN-LIKE AND PLATELET-DERIVED GROWTH-FACTOR GENES IN HUMAN UTERINE TISSUES [J].