PREPARATION AND CHARACTERIZATION OF A SERIES OF 35S-LABELLED ARYL SULPHATE ESTERS FOR METABOLIC STUDIES

In order to provide a series of suitable compounds for metabolic studies, methods were developed for the preparation of crystalline potassium salts of the 35S-labelled sulphate esters of 4-hydroxybiphenyl, 4-cyclohexylphenol, 2-cyclohexylphenol, 2-naphthol and 5,6,7,8-tetrahydro 2-naphthol in good yield. Infrared spectrophotometric analysis of the products showed strong absorption bands in the regions 1280-1220 cm-1 and 1070-1040 cm-1 characteristic of the ester sulphate grouping. Ultraviolet absorption spectra revealed a characteristic spectral shift from the absorption region associated with the parent phenols (270-310 mμ) to that associated with sulphated phenols (240-280 mμ). © 1969.