EFFICIENT GENE INTRODUCTION INTO RICE BY ELECTROPORATION AND ANALYSIS OF TRANSGENIC PLANTS - USE OF ELECTROPORATION BUFFER LACKING CHLORIDE-IONS

被引：98

作者：

TADA, Y

SAKAMOTO, M

FUJIMURA, T

机构：

[1] Plant Biotechnology Department, Life Science Institute, Mitsui Toatsu Chemicals, Inc, Mobara, 297

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 04期

关键词：

β-Glucuronidase; Co-transformation; Electroporation; Hygromycin phosphotransferase; Rice;

D O I：

10.1007/BF00226748

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We have developed a method for reproducibly obtaining transgenic rice at a high frequency (10-4): electroporation with a buffer in which chloride ions are replaced with organic acids. Co-transformation frequencies of the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes located on two separate plasmids were higher than 50%. Transgenic rice plants contained multiple copies of introduced genes integrated into their genomes in a complex manner. GUS enzyme activity was not proportional to gene copy number. Introduced HPT genes were detected and expressed in the progeny of transformants. © 1990 Springer-Verlag.

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页码：475 / 480

页数：6

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