INFLUENCE OF DIFFUSION ON EXCITATION-ENERGY TRANSFER IN SOLUTIONS BY GIGAHERTZ HARMONIC CONTENT FREQUENCY-DOMAIN FLUOROMETRY

被引：17

作者：

LAKOWICZ, JR ^{[1
]}

SZMACINSKI, H ^{[1
]}

GRYCZYNSKI, I ^{[1
]}

WICZK, W ^{[1
]}

JOHNSON, ML ^{[1
]}

机构：

[1] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908

来源：

JOURNAL OF PHYSICAL CHEMISTRY | 1990年 / 94卷 / 22期

关键词：

D O I：

10.1021/j100385a011

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the effects of diffusion on fluorescence resonance energy transfer, as observed by using the frequency-domain technique. Energy transfer between indole (donor) and dansylamide (acceptor) was studied in low and high viscosity solutions. At the current level of resolution, the donor fluorescence decays were satisfactorily analyzed in terms of the theoretical function including translational diffusion proposed by Gosele et al. (Chem. Phys. Lett. 1975, 24, 519). The Forster equation, which does not consider diffusion, was found to significantly overestimate the critical transfer distance (R0) in fluid solutions and to provide an inadequate representation of the data. These results demonstrate the ability to determine mutual diffusion coefficients from the frequency response of the donor emission. © 1990 American Chemical Society.

引用

页码：8413 / 8416

页数：4

共 23 条

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