THE PRIMARY STRUCTURE OF RAT RIBOSOMAL-PROTEINS - THE AMINO-ACID-SEQUENCES OF L27A AND L28 AND CORRECTIONS IN THE SEQUENCES OF S4 AND S12

被引：25

作者：

WOOL, IG

CHAN, YL

PAZ, V

OLVERA, J

机构：

[1] Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1050卷 / 1-3期

关键词：

(Rat); Protein amino acid sequence; Ribosomal proteins L27a; Ribosomal proteins L28; Ribosomal proteins S12; Ribosomal proteins S4;

D O I：

10.1016/0167-4781(90)90143-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amino acid sequences of rat ribosomal proteins L27a and L28 were deduced from the sequences of nucleotides in recombinant cDNAs and confirmed from the NH2-terminal amino acid sequences of the proteins. L27a contains 147 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 16 476. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 18-22 copies of the L27a gene. The mRNA for the protein is about 600 nucleotides in length. L27a is homologous to mouse L27a (there are 3 amino acid changes) and to yeast L29. Rat ribosomal protein L28 has 136 amino acids (its NH2-terminal methionine is also processed after translation) and has a molecular weight of 15 707. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 9 or 10 copies of the L28 gene. The mRNA for the protein is about 640 nucleotides in length. L28 contains a possible internal duplication of 9 residues. Corrections are recorded in the sequences reported before for rat ribosomal proteins S4 and S12. © 1990.

引用

页码：69 / 73

页数：5

共 13 条

[1] CLONING AND SEQUENCING OF MOUSE RIBOSOMAL-PROTEIN S12 CDNA [J].

AYANE, M ;

NIELSEN, P ;

KOHLER, G .

NUCLEIC ACIDS RESEARCH, 1989, 17 (16) :6722-6722

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].

BEAUCAGE, SL ;

CARUTHERS, MH .

TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862

[3] ISOLATION AND CHARACTERIZATION OF A MURINE CDNA CLONE HIGHLY HOMOLOGOUS TO THE YEAST L29 RIBOSOMAL-PROTEIN GENE [J].

BELHUMEUR, P ;

PATERNO, GD ;

BOILEAU, G ;

CLAVERIE, JM ;

SKUP, D .

NUCLEIC ACIDS RESEARCH, 1987, 15 (03) :1019-1029

[4]

CHAN YL, 1987, J BIOL CHEM, V262, P12879

[5]

CHAN YL, 1988, J BIOL CHEM, V263, P2891

[6]

DAYHOFF MO, 1978, ATLAS PROTEIN SEQUEN, V5, P1

[7]

DEVI KRG, 1989, BIOCHIM BIOPHYS ACTA, V1008, P258

[8] SEQUENCE DETERMINANTS OF CYTOSOLIC N-TERMINAL PROTEIN PROCESSING [J].

FLINTA, C ;

PERSSON, B ;

JORNVALL, H ;

VONHEIJNE, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 154 (01) :193-196

[9] THE PRIMARY STRUCTURE OF RAT RIBOSOMAL-PROTEIN S10 [J].

GLUCK, A ;

CHAN, YL ;

LIN, A ;

WOOL, IG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (01) :105-109

[10]

KAUFER NF, 1983, NUCLEIC ACIDS RES, V11, P3123

← 1 2 →