PARTIAL-PURIFICATION AND PROPERTIES OF THE SESQUITERPENE BETA-SELINENE CYCLASE FROM CITROFORTUNELLA-MITIS FRUITS

被引：12

作者：

BELINGHERI, L ^{[1
]}

CARTAYRADE, A ^{[1
]}

PAULY, G ^{[1
]}

GLEIZES, M ^{[1
]}

机构：

[1] UNIV BORDEAUX 1,PHYSIOL CELLULAIRE VEGETALE LAB,CNRS,URA 568,AVE FAC,F-33405 TALENCE,FRANCE

来源：

PLANT SCIENCE | 1992年 / 84卷 / 02期

关键词：

CITROFORTUNELLA-MITIS; SESQUITERPENE; SELINENE CYCLASE; DEAE SEPHACEL PURIFICATION; FARNESYL DIPHOSPHATE;

D O I：

10.1016/0168-9452(92)90126-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From a supernatant fraction of the periPheral flavedo of immature calamondin (Citrofortunella mitis) fruits were extracted by sesquiterpene cyclase catalyzing the conversion of farnesyl diphosphate to beta-selinene. This enzyme was partially purified by ion exchange chromatography and its properties were investigated. The molecular weight was estimated to be in the range of 67 000 by gel filtration. The K(m) for the substrate farnesyl diphosphate was found to be 45-mu-M. This protein exhibited an isoelectric point of 6.0. It required the presence of divalent metal cations and Mg2+ was preferred to Mn2+.

引用

页码：129 / 136

页数：8

共 34 条

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