RESTRICTION-FRAGMENT MAP OF THE TEMPERATE BACILLUS-SUBTILIS BACTERIOPHAGE-SP02

The endonucleases BglI, BglII, EcoRI, SalI, SmaI, and XbaI were used to fragment the phage SP02 DNA. Electrophoretic analysis using ethidiumbromide agarose gels showed the phage to have nine BglI sites, one BglII site, four EcoRI sites, one SalI site, one SmaI site, and six XbaI sites. Using partial digestions, multiple endonuclease digestion, and autoradiography the fragments were sized and ordered into a circular map of 23 Md. Such an analysis locates the endonuclease sites, indicates which endonucleases are potentially useful in cloning with SP02, and allows insertions and/or deletions in the SP02 DNA to be characterized. © 1979.