ELECTROCHEMICAL RESPONSE OF AN ENZYME-LOADED POLYANILINE FILM

A method is reported for the electrochemical preparation of a glucose oxidase loaded polyaniline film. The current response of the film in the presence of glucose was recorded at a constant potential and under cyclic linear potential sweep. An aerobic current due to hydrogen peroxide could be recorded and an anaerobic signal, which was a function of time and glucose concentration, could be measured at +0.65 V as a current increasing linearly with time. Attempts to elucidate the source and nature of this behaviour through the study of the response of the enzyme-loaded film at different thicknesses indicated that the charge transport limitation present in glucose-free solutions diminished with time in the presence of glucose. The 'species' resulting in this mediation could not be unambiguously identified.