GAS-CHROMATOGRAPHY MASS-SPECTROMETRY ANALYSIS OF 2-AMINO-1-METHYL-6-PHENYL-IMIDAZO[4,5-B]PYRIDINE IN URINE AND FECES

被引：14

作者：

FRIESEN, MD ^{[1
]}

GARREN, L ^{[1
]}

BEREZIAT, JC ^{[1
]}

KADLUBAR, F ^{[1
]}

LIN, DX ^{[1
]}

机构：

[1] NATL CTR TOXICOL RES,JEFFERSON,AR 72079

来源：

ENVIRONMENTAL HEALTH PERSPECTIVES | 1993年 / 99卷

关键词：

D O I：

10.2307/3431476

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 mug dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.

引用

页码：179 / 181

页数：3

共 5 条

[1] INDUCTION OF LYMPHOMA IN CDF1 MICE BY THE FOOD MUTAGEN, 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE [J].