PURIFICATION OF THE LYSR FAMILY REGULATOR, CLCR, AND ITS INTERACTION WITH THE PSEUDOMONAS-PUTIDA CLCABD CHLOROCATECHOL OPERON PROMOTER

被引：36

作者：

COCO, WM ^{[1
]}

PARSEK, MR ^{[1
]}

CHAKRABARTY, AM ^{[1
]}

机构：

[1] UNIV ILLINOIS, COLL MED, DEPT MICROBIOL & IMMUNOL, CHICAGO, IL 60612 USA

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 17期

关键词：

D O I：

10.1128/JB.176.17.5530-5533.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.

引用

页码：5530 / 5533

页数：4

共 21 条

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