DIHAPLOID PLANTS OF ROSES (ROSA X HYBRIDA, CV SONIA) OBTAINED BY PARTHENOGENESIS INDUCED USING IRRADIATED POLLEN AND IN-VITRO CULTURE OF IMMATURE SEEDS

被引:18
作者
MEYNET, J
BARRADE, R
DUCLOS, A
SIADOUS, R
机构
[1] INRA, Station d'Amélioration des Plantes Florales, La Gaudine
[2] Commissariat à l'Énergie Atomique, Service de Radioagronomie, CEN de Cadarache
来源
AGRONOMIE | 1994年 / 14卷 / 03期
关键词
HAPLOIDY; ROSA X HYBRIDA; POLLEN IRRADIATION; GAMMA-RAYS;
D O I
10.1051/agro:19940303
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
'Sonia' var rose ovules produced plants through in vitro culture after being pollinated with irradiated (gamma rays) pollen. A 500-Gy minimum dose was sufficient to inactivate pollen and induce in situ parthenogenesis; in vitro culture was necessary for embryo rescue. The dihaploid plants originated from small embryos which occupied only a part of the carpel cavity; they were clearly distinguished from tetraploid plants by miniaturization of all organs. The dihaploid plants were observed under glass-house conditions until flowering presented a normal gynoecium. They produced a small amount of pollen of reduced but regular size; growth and development were faster than the tetraploid controls in summer.
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页码:169 / 175
页数:7
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