Channel crosstalk due to fiber four-wave mixing (FWM) in multichannel systems operated around the zero-dispersion wavelength is experimentally studied. After determining the wavelength at which FWM light is most efficiently generated, the FWM efficiency is measured for possible frequency combinations which generate FWM light at that wavelength. Using these data, FWM crosstalk in multiwavelength systems is evaluated. The results show that actual crosstalk is less than the value estimated by the theoretical model assuming the uniformly distributed chromatic dispersion for 80-km-long fibers. It is concluded that the theoretical model can be applied to system design dealing with the worst condition.