CLONING AND SEQUENCING OF THE PAC GENE ENCODING THE PENICILLIN-G ACYLASE OF BACILLUS-MEGATERIUM ATCC-14945

被引：40

作者：

MARTIN, L ^{[1
]}

PRIETO, MA ^{[1
]}

CORTES, E ^{[1
]}

GARCIA, JL ^{[1
]}

机构：

[1] CSIC,CTR INVEST BIOL,DEPT MOLEC MICROBIOL,E-28006 MADRID,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 125卷 / 2-3期

关键词：

PENICILLIN ACYLASE; BACILLUS MEGATERIUM; ESCHERICHIA COLI; PAC GENE;

D O I：

10.1016/0378-1097(94)00510-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 (proA, leuB) using a selective minimal medium containing phenylacetyl-L-leucine instead of L-leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other beta-lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.

引用

页码：287 / 292

页数：6

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