DETECTION OF MEMBRANE AND SOLUBLE INTERLEUKIN-6 RECEPTOR IN LYMPHOID MALIGNANCIES

被引：36

作者：

LAVABREBERTRAND, T ^{[1
]}

EXBRAYAT, C ^{[1
]}

LIAUTARD, J ^{[1
]}

GAILLARD, JP ^{[1
]}

BASKEVITCH, PP ^{[1
]}

POUJOL, N ^{[1
]}

DUPERRAY, C ^{[1
]}

BOURQUARD, P ^{[1
]}

BROCHIER, J ^{[1
]}

机构：

[1] INSERM,U291,MONTPELLIER,FRANCE

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1995年 / 91卷 / 04期

关键词：

INTERLEUKIN-6; RECEPTOR; CHRONIC LYMPHOCYTIC LEUKEMIA; NON-HODGKINS LYMPHOMA;

D O I：

10.1111/j.1365-2141.1995.tb05403.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied the membrane expression of the gp80 chain of IL-6 receptor (IL-GR) by quantitative flow cytometry in chronic lymphocytic leukaemia (CLL) and leukaemic centrocytic lymphoma using a panel of seven monoclonal antibodies, IL-6R was detected in 18/26 CLL cases and 4/7 lymphoma cases, with a mean antigen density < 3000 molecules/cell. Multiple labelling experiments confirmed the IL-GR expression by neoplastic cells, Specific mRNA was found by RT-PCR in neoplastic cells, A specific ELISA test was designed using two anti-IL-6 receptor MAbs to measure the serum soluble IL-GR (sIL-6R) in CLL (n = 48), B-cell non-Hodgkin's lymphoma (NHL; n = 40), and monoclonal gammopathy (MG; n = 32). SIL-6R was higher in CLL (170 +/- 12.6 ng/ml) in NHL (160 +/- 12 ng/ml) and MG patients (183 +/- 23 ng/ml) than in age-matched controls (100 +/- 5.6 ng/ml; P < 0.001) and higher in high-grade than low-grade NHL, No correlation was noted with a previous treatment. Among CLL, cases the patients classified as stage B according to the Binet's staging of the disease had the highest sIL-6R values, thus suggesting a link with tumour cell mass.

引用

页码：871 / 877

页数：7

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