A PLASMID ENCODING ENZYMES FOR NYLON OLIGOMER DEGRADATION - NUCLEOTIDE-SEQUENCE AND ANALYSIS OF POAD2

被引：26

作者：

KATO, K ^{[1
]}

OHTSUKI, K ^{[1
]}

KODA, Y ^{[1
]}

MAEKAWA, T ^{[1
]}

YOMO, T ^{[1
]}

NEGORO, S ^{[1
]}

URABE, I ^{[1
]}

机构：

[1] OSAKA UNIV,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

NYLON OLIGOMER; DEGRADATIVE PLASMID; ENZYME EVOLUTION;

D O I：

10.1099/13500872-141-10-2585

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The entire nucleotide sequence of nylon oligomer degradative plasmid pOAD2 from Flavobacterium sp. KI723T1 was determined. pOAD2 comprises 45519 bp, with a 66.6 mol% G+C content. The precise loci of the four nylon oligomer degradation genes, namely nylA (6-aminohexanoate-cyclic-dimer hydrolase gene), nylB (6-aminohexanoate-dimer hydrolase), nylB' (a gene having 88% homology to nylB) and nylC (endo-type 6-aminohexanoate oligomer hydrolase), and five IS6100 elements were identified on this plasmid. Comparison of the sequence of pOAD2 with those in the GenBank and EMBL databases revealed that the deduced amino acid sequences from eight regions of pOAD2 had significant similarity with the sequences of gene products such as oppA-F (oligopeptide permeases), ftsX (filamentation temperature sensitive), penDE (isopenicillin N-acyltransferase) and rep (plasmid incompatibility). A functional map of pOAD2 is presented.

引用

页码：2585 / 2590

页数：6

共 31 条

[1] THE AMI LOCUS OF THE GRAM-POSITIVE BACTERIUM STREPTOCOCCUS-PNEUMONIAE IS SIMILAR TO BINDING PROTEIN-DEPENDENT TRANSPORT OPERONS OF GRAM-NEGATIVE BACTERIA [J].