INACTIVATION OF LIPID-CONTAINING VIRUSES BY HYDROPHOBIC PHOTOSENSITIZERS AND NEAR-ULTRAVIOLET RADIATION

被引:30
作者
SNIPES, W
KELLER, G
WOOG, J
VICKROY, T
DEERING, R
KEITH, A
机构
[1] Biophysics Laboratory, Department of Biochemistry and Biophysics, The Pennsylvania State University, University Park, Pennsylvania
关键词
D O I
10.1111/j.1751-1097.1979.tb07767.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abstract—The hydrophobic photosensitizers acridine and phenothiazine inactivate the lipid‐contnining viruses PM2,φ6, and herpes simplex when samples are illuminated with near‐UV radiation. φ23–1‐a. which is insensitive to organic solvents and presumably contains no lipids. is not inactivated under comparable conditions. For acridinc, the inactivation of virus requires that oxygen be present and is inhibited by sodium azide, implicating the involvement of singlet oxygen. For phenothiazine, oxygen is not required for photosensitized inactivation. Treatment of PM2 with acridine and near‐UV light caused a complete disruption of the virion, as determined by sucrose gradient analysis of treated and untreated samples. These data and related observations suggest that lipid‐containing viruses are inactivated through photosensitized membrane damage. Copyright © 1979, Wiley Blackwell. All rights reserved
引用
收藏
页码:785 / 790
页数:6
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