PNEUMOCYSTIS-CARINII - NEW SEPARATION METHOD FROM LUNG-TISSUE

被引：55

作者：

WALZER, PD

RUTLEDGE, ME

YONEDA, K

STAHR, BJ

机构：

[1] UNIV KENTUCKY,COLL MED,DEPT MED,DIV INFECT DIS,LEXINGTON,KY 40507

[2] UNIV KENTUCKY,COLL MED,DEPT PATHOL,LEXINGTON,KY

来源：

EXPERIMENTAL PARASITOLOGY | 1979年 / 47卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Centrifugation; Collagenase (EC 3.4.243); density gradient; digestive; Electron microscopy; Enzyme; Ficoll-Hypaque; Hyaluronidase; (EC; 3.2.1.35; 3.2.1.36; 4.2.1.36); Isolation and purification; Lung; Parasitic; Pneumocystis carinii; Protozoa;

D O I：

10.1016/0014-4894(79)90088-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A new method of separating Pneumocystis carinii from infected rat, human, and mouse lung tissue has been developed, based, in part, on techniques used for the separation of lymphocytes from hematopoietic and lymphoid organs. The lungs are homogenized with a Teflon pestle and fine wire-mesh screen, digested with collagenase and hyaluronidase, and centrifuged on a discontinuous Ficoll-Hypaque density gradient. The method produces no morphologic alterations in P. carinii, as judged by light and electron microscopy. The method has been adapted for the quantitation of P. carinii in tissues and the production of antisera in rabbits. © 1979.

引用

页码：356 / 368

页数：13

共 29 条

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