DETERMINATION OF ALBUTEROL CONCENTRATIONS IN HUMAN PLASMA USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

A fast, simple, and accurate method for determining albuterol concentrations in human plasma has been developed and validated for use in routine clinical analyses. This method involves a solid-phase extraction procedure using silica cartridges and normal-phase high-performance liquid chromatography with fluorescence detection. Regression analysis showed the method was linear over the standard curve range 1-16 ng ml-1. The percent recovery for albuterol and the internal standard, bamethane, at 5 ng ml-1 was found to be ≫90%. The newly developed method has been applied in the analysis of plasma samples from patients and healthy volunteers. © 1990.