REDUCED LEVELS OF ALPHA-1 (I) COLLAGEN MESSENGER-RNA IN CELLS IMMORTALIZED BY MUTANT P53 OR TRANSFORMED BY RAS

被引：4

作者：

EIZENBERG, O ^{[1
]}

OREN, M ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT CHEM IMMUNOL, IL-76100 REHOVOT, ISRAEL

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1129卷 / 01期

关键词：

P53; RAS; COLLAGEN; TRANSFORMATION; TUMOR SUPPRESSOR;

D O I：

10.1016/0167-4781(91)90209-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genes whose expression patterns are altered in a cell line immortalized by mutant p53 were isolated by differential screening of a cDNA library. Levels of alpha-1 (I) collagen mRNA were reduced in the majority of immortalized cell lines which greatly overproduced the transfected mutant p53. This may reflect a co-selection during the establishment of the cell lines, rather than a direct effect of p53 on alpha-1 (I) collagen gene expression. On the other hand, a more direct relationship could be demonstrated between the expression of activated ras and a reduction in alpha-1 (I) collagen mRNA. Such reduction could partially account for the effects of ras on cell shape and cell proliferation.

引用

页码：34 / 42

页数：9

共 53 条

[1]

ALLENHOFFMANN BL, 1990, J BIOL CHEM, V265, P5219

[2] COTRANSFECTION OF GRANULOSA-CELLS WITH SIMIAN VIRUS-40 AND HA-RAS ONCOGENE GENERATES STABLE LINES CAPABLE OF INDUCED STEROIDOGENESIS [J].

AMSTERDAM, A ;

ZAUBERMAN, A ;

MEIR, G ;

PINHASIKIMHI, O ;

SUH, BS ;

OREN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7582-7586

[3] CHROMOSOME-17 DELETIONS AND P53 GENE-MUTATIONS IN COLORECTAL CARCINOMAS [J].

BAKER, SJ ;

FEARON, ER ;

NIGRO, JM ;

HAMILTON, SR ;

PREISINGER, AC ;

JESSUP, JM ;

VANTUINEN, P ;

LEDBETTER, DH ;

BARKER, DF ;

NAKAMURA, Y ;

WHITE, R ;

VOGELSTEIN, B .

SCIENCE, 1989, 244 (4901) :217-221

[4] MECHANISMS OF KIRSTEN MURINE SARCOMA-VIRUS TRANSFORMATION-INDUCED CHANGES IN THE COLLAGEN PHENOTYPE AND SYNTHETIC RATE OF BALB 3T3 CELLS [J].

BATEMAN, JF ;

PETERKOFSKY, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6028-6032

[5]

BORNSTEIN P, 1989, PROG NUCLEIC ACID RE, V37, P67

[6] REGULATION OF METALLOTHIONEIN THYMIDINE KINASE FUSION PLASMIDS INJECTED INTO MOUSE EGGS [J].

BRINSTER, RL ;

CHEN, HY ;

WARREN, R ;

SARTHY, A ;

PALMITER, RD .

NATURE, 1982, 296 (5852) :39-42

[7] CLONING AND SEQUENCING OF A DEOXYRIBONUCLEIC-ACID COPY OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MESSENGER RIBONUCLEIC-ACID ISOLATED FROM CHICKEN MUSCLE [J].

DUGAICZYK, A ;

HARON, JA ;

STONE, EM ;

DENNISON, OE ;

ROTHBLUM, KN ;

SCHWARTZ, RJ .

BIOCHEMISTRY, 1983, 22 (07) :1605-1613

[8] PARTICIPATION OF P53 CELLULAR TUMOR-ANTIGEN IN TRANSFORMATION OF NORMAL EMBRYONIC-CELLS [J].

ELIYAHU, D ;

RAZ, A ;

GRUSS, P ;

GIVOL, D ;

OREN, M .

NATURE, 1984, 312 (5995) :646-649

[9] OVERPRODUCTION OF P53 ANTIGEN MAKES ESTABLISHED CELLS HIGHLY TUMORIGENIC [J].

ELIYAHU, D ;

MICHALOVITZ, D ;

OREN, M .

NATURE, 1985, 316 (6024) :158-160

[10] WILD-TYPE P53 CAN INHIBIT ONCOGENE-MEDIATED FOCUS FORMATION [J].

ELIYAHU, D ;

MICHALOVITZ, D ;

ELIYAHU, S ;

PINHASIKIMHI, O ;

OREN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8763-8767

← 1 2 3 4 5 6 →