CHROMOSOMAL ASSIGNMENT OF GENES IN BARLEY USING TELOSOMIC WHEAT BARLEY ADDITION LINES

被引：7

作者：

CANNELL, M

KARP, A

ISAAC, PG

SHEWRY, P

机构：

[1] UNIV BRISTOL,DEPT AGR SCI,AFRC,INST ARABLE CROPS RES,LONG ASHTON RES STN,BRISTOL BS18 9AF,AVON,ENGLAND

[2] NICKERSON INT SEED CO LTD,CAMBRIDGE CB4 4WE,ENGLAND

来源：

GENOME | 1992年 / 35卷 / 01期

关键词：

GENE MAPPING; TELOSOMIC ADDITIONS; CDNA CLONES; WHEAT-BARLEY; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS;

D O I：

10.1139/g92-003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Characterized DNA clones have been used as probes in Southern blotting and hybridization analysis of genomic DNA from a set of wheat-barley telosomic addition lines to assign genes to chromosome arms in barley (Hordeum vulgare). The lines used included a newly available addition line with the short arm of chromosome 5. Addition lines with the long arm or the whole of chromosome 5 of H. vulgare are not available, so addition lines with chromosomes 5 and 5S of a related wild species, Hordeum chilense, were also used. A total of 23 characterized cDNAs and two characterized genomic clones were mapped to chromosome arms in this way.

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页码：17 / 23

页数：7

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