EFFECTS OF LYMPHOCYTE SUBPOPULATIONS ON THE CLONAL ASSAY OF HPRT MUTANTS - OCCUPATIONAL EXPOSURE TO CYTOSTATIC DRUGS

被引：10

作者：

DUBEAU, H

ZAZI, W

BARON, C

MESSING, K

机构：

[1] CINBIOSE, Département des Sciences Biologiques, Université du Québec à Montréal, C.P. 8888, Succursale A, Montréal

来源：

MUTATION RESEARCH | 1994年 / 321卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHEMOTHERAPY; HPRT(-) MUTANT FREQUENCY; CLONING EFFICIENCY; HPRT(-) MUTANTS; T-LYMPHOCYTES SUBSETS;

D O I：

10.1016/0165-1218(94)90039-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mutagenic effect of occupational exposure to antineoplastic agents was studied in chemotherapy nurses and pharmacists using the T-lymphocyte clonal assay. A significant increase in mutant frequency was observed compared to controls. However, in the present study, cloning efficiency without selection (CE(U)) was significantly reduced in exposed personnel raising the possibility of an overestimation of the calculated MF. Changes in lymphocyte populations and clonal potential of T-cells were also observed following exposure. CE(U) was related to % CD4 cells but CE with selection (CE(TG)) was not. Differences in clonal ability of T-cells under selective and unselective conditions coupled with differential lethal effect of antineoplastic agents on lymphocyte subsets may result in inaccurate estimation of MF.

引用

页码：147 / 157

页数：11

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