GENOMIC FINGERPRINTING OF CLOSTRIDIUM-DIFFICILE ISOLATES BY USING A RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ASSAY

被引：40

作者：

BARBUT, F

MARIO, N

DELMEE, M

GOZIAN, J

PETIT, JC

机构：

[1] UNIV CATHOLIQUE LOUVAIN,UNITE MICROBIOL,B-1200 BRUSSELS,BELGIUM

[2] HOP ST ANTOINE,COMMUN BIOL MOLEC LAB,F-75571 PARIS 12,FRANCE

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 114卷 / 02期

关键词：

CLOSTRIDIUM-DIFFICILE; RAPD ASSAY; GENETIC MARKER;

D O I：

10.1016/0378-1097(93)90513-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study describes the use of a new and easy method called random amplified polymorphic DNA (RAPD) assay to distinguish strains of C. difficile. We used two single short primers (AP4 and AP5) with arbitrary nucleotide sequences in a polymerase chain reaction to amplify genomic DNA. The profiles observed after electrophoretic separation were able to distinguish 20 reference C difficile strains previously serotyped by Delmee's method. The fingerprints of 11 epidemiologically unrelated C. difficile strains clearly yielded a DNA polymorphism between all the strains. Latterly, RAPD profiles of 11 C difficile strains isolated from 2 independant suspected outbreaks showed, in each case, a predominant banding pattern correponding to an epidemic strain. These results suggest that RAPD assay could be a valuable tool for epidemiological studies.

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页码：161 / 166

页数：6

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