IDENTIFICATION OF 50-KDA SNAKE-VENOM PROTEINS WHICH SPECIFICALLY INHIBIT PLATELET-ADHESION TO COLLAGEN

被引：29

作者：

SMITH, JB

DANGELMAIER, C

SELAK, M

机构：

[1] TEMPLE UNIV,HLTH SCI CTR,DEPT PHARMACOL,PHILADELPHIA,PA 19140

[2] TEMPLE UNIV,HLTH SCI CTR,THROMBOSIS RES CTR,PHILADELPHIA,PA 19140

来源：

FEBS LETTERS | 1991年 / 283卷 / 02期

关键词：

ADHESION; COLLAGEN; PLATELET; SNAKE VENOM;

D O I：

10.1016/0014-5793(91)80615-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Of 32 snake venoms tested, the crude venoms of four (B. atrox, B. jararaca, A. halys blomhoffii and C. basiliscus) showed strong inhibitory activity in an assay of platelet adhesion to collagen. Active 50 kDa proteins were purified to homogeneity from each venom and found to be rich in cysteine on amino acid analysis. A monoclonal antibody raised against the purified B. atrox protein crossreacted strongly with the 50 kDa proteins from B. jararaca and A. halys blomhoffi and weakly with the protein from C. basiliscus, indicating that all four proteins possess a similar epitope. The proteins inhibited platelet aggregation induced by collagen but not by other agonists.

引用

页码：307 / 310

页数：4

共 15 条

[1] THROMBOXANES - NEW GROUP OF BIOLOGICALLY-ACTIVE COMPOUNDS DERIVED FROM PROSTAGLANDIN ENDOPEROXIDES [J].