DEMONSTRATION OF CONCURRENT DENGUE-1 AND DENGUE-3 INFECTION IN 6 PATIENTS BY THE POLYMERASE CHAIN-REACTION

被引：52

作者：

LAILLE, M ^{[1
]}

DEUBEL, V ^{[1
]}

SAINTEMARIE, FF ^{[1
]}

机构：

[1] INST PASTEUR, ARBOVIRUS LAB, F-75724 PARIS 15, FRANCE

来源：

JOURNAL OF MEDICAL VIROLOGY | 1991年 / 34卷 / 01期

关键词：

DUAL DENGUE INFECTION; GENOMIC AMPLIFICATION; VIRUS DIAGNOSIS;

D O I：

10.1002/jmv.1890340109

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A dual viremia resulting from naturally acquired dengue 1 and dengue 3 infections in six patients experiencing a dengue-like syndrome during the epidemic in New Caledonia in 1989 is reported. Serotype identification was first based on virus isolation in mosquito cells and immunofluorescence using type-specific monoclonal antibodies. The double infection was confirmed directly in blood samples by the polymerase chain reaction (PCR) on genomic RNA and hybridization of the amplified cDNA fragments with type-specific DNA probes.

引用

页码：51 / 54

页数：4

共 13 条

[1] ANTIBODY CAPTURE ELISA FOR IGM ANTIBODY TITRATION IN SERA FOR DENGUE SERODIAGNOSIS AND SURVEILLANCE [J].