COMPARATIVE METABOLIC PROFILING OF CHLORAMPHENICOL BY ISOLATED HEPATOCYTES FROM RAT AND TROUT (ONCORHYNCHUS-MYKISS)

被引：7

作者：

CRAVEDI, JP

BARADAT, M

机构：

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1991年 / 100卷 / 03期

关键词：

D O I：

10.1016/0742-8413(91)90055-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Isolated liver cells from rat and rainbow trout were used to study the biotransformation of labelled chloramphenicol (CP). 2. The H-3-CP metabolic rates were 1.7 nmol/hr/10(6) rat hepatocytes and 0.2 nmol/hr/10(6) trout hepatocytes. 3. In rat, as in trout liver cells, the major metabolite was CP-glucuronide. In addition to this conjugate, CP-base, CP-alcohol and CP-oxamic acid were detected in significant amounts. 4. These results are compared with in vivo data on CP metabolism previously obtained in our laboratory in fish and mammals.

引用

页码：649 / 652

页数：4

共 18 条

[1] SPECTRAL PROPERTIES OF SUBSTRATE CYTOCHROME-P-450 INTERACTION AND CATALYTIC ACTIVITY OF XENOBIOTIC METABOLIZING ENZYMES IN ISOLATED RAINBOW-TROUT LIVER-CELLS [J].