LARGE-SCALE EXTRACTION OF ALPHA-LACTALBUMIN AND BETA-LACTOGLOBULIN FROM BOVINE WHEY BY PRECIPITATION WITH POLYETHYLENE-GLYCOL AND PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS

被引：7

作者：

ORTIN, A ^{[1
]}

CEBRIAN, JA ^{[1
]}

JOHANSSON, G ^{[1
]}

机构：

[1] UNIV ZARAGOZA,FAC VET,DEPT BIOCHEM & CELLULAR MOLEC BIOL,E-50013 ZARAGOZA,SPAIN

来源：

PREPARATIVE BIOCHEMISTRY | 1992年 / 22卷 / 01期

关键词：

D O I：

10.1080/10826069208018019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The milk proteins alpha-lactalbumin and beta-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. The possible use of this combination for large scale purification of these whey proteins is discussed.

引用

页码：53 / 66

页数：14

共 33 条

[1]

Albertsson P., 1986, PARTITION CELL PARTI

[2]

Albertsson P A, 1982, Methods Biochem Anal, V28, P115, DOI 10.1002/9780470110485.ch2

[3] IMPROVED METHOD FOR THE PREPARATION OF CRYSTALLINE BETA-LACTOGLOBULIN AND ALPHA-LACTALBUMIN FROM COWS MILK [J].

ASCHAFFENBURG, R ;

DREWRY, J .

BIOCHEMICAL JOURNAL, 1957, 65 (02) :273-277

[4] IMMUNOLOGICAL STUDIES OF MODIFIED AND ENZYMICALLY DEGRADED BETA-LACTOGLOBULIN AND ALPHA-LACTALBUMIN [J].

BENGTSSON, C ;

HANSON, LA ;

JOHANSSON, BG .

ACTA CHEMICA SCANDINAVICA, 1962, 16 (01) :127-&

[5]

DEVILLEGAS MCD, 1987, MILCHWISSENSCHAFT, V42, P357

[6] ISOLATION OF CRYSTALLINE ALPHA-LACTALBUMIN FROM MILK [J].

GORDON, WG ;

SEMMETT, WF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1953, 75 (02) :328-330

[7]

GORNALL AG, 1949, J BIOL CHEM, V177, P751

[8] PREPARATION OF SOME IRON-BINDING PROTEINS AND ALPHA-LACTALBUMIN FROM BOVINE MILK [J].

GROVES, ML .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 100 (01) :154-&

[9] SEPARATION OF MILK PROTEIN ON DEXTRAN GEL [J].

HILL, RD ;

HANSEN, RR .

JOURNAL OF DAIRY RESEARCH, 1964, 31 (03) :291-&

[10] ALCOHOL OXIDASE A FLAVOPROTEIN FROM SEVERAL BASIDIOMYCETES SPECIES - CRYSTALLIZATION BY FRACTIONAL PRECIPITATION WITH POLYETHYLENE GLYCOL [J].

JANSSEN, FW ;

RUELIUS, HW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 151 (02) :330-&

← 1 2 3 4 →