THE EFFECT OF SALT ON THE MICHAELIS MENTEN CONSTANT OF THE HIV-1 PROTEASE CORRELATES WITH THE HOFMEISTER SERIES

被引：69

作者：

WONDRAK, EM ^{[1
]}

LOUIS, JM ^{[1
]}

OROSZLAN, S ^{[1
]}

机构：

[1] NCI, DIV CANC BIOL & DIAG, BETHESDA, MD 20892 USA

来源：

FEBS LETTERS | 1991年 / 280卷 / 02期

关键词：

HIV-1; PROTEASE; ENZYME KINETICS; SALT EFFECT; HOFMEISTER SERIES;

D O I：

10.1016/0014-5793(91)80327-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of different types of salt on the proteolytic activity of HIV-1 protease was studied. At a similar ionic strength, the enzyme activity changed according to the salting out effect of the ions used (Hofmeiser series). Kinetic studies showed that a stronger salting out effect of the ions rather than the higher ionic strength per se increased the affinity to the substrate (K(m)) but in general did not alter the K(cat) value.

引用

收藏

页码：344 / 346

页数：3

相关论文

共 19 条

[1] PURIFICATION, ASSAY AND KINETIC FEATURES OF HIV-1 PROTEINASE [J].

BILLICH, A ;

HAMMERSCHMID, F ;

WINKLER, G .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1990, 371 (03) :265-272

[2] A DELETION MUTATION IN THE 5' PART OF THE POL GENE OF MOLONEY MURINE LEUKEMIA-VIRUS BLOCKS PROTEOLYTIC PROCESSING OF THE GAG AND POL POLYPROTEINS [J].

CRAWFORD, S ;

GOFF, SP .

JOURNAL OF VIROLOGY, 1985, 53 (03) :899-907

[3] SELECTIVE PHOSPHINATE TRANSITION-STATE ANALOG INHIBITORS OF THE PROTEASE OF HUMAN-IMMUNODEFICIENCY-VIRUS [J].

GROBELNY, D ;

WONDRAK, EM ;

GALARDY, RE ;

OROSZLAN, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 169 (03) :1111-1116

[4]

KATOH I, 1985, VIROLOGY, V145, P289

[5] ACTIVE HUMAN IMMUNODEFICIENCY VIRUS PROTEASE IS REQUIRED FOR VIRAL INFECTIVITY [J].

KOHL, NE ;

EMINI, EA ;

SCHLEIF, WA ;

DAVIS, LJ ;

HEIMBACH, JC ;

DIXON, RAF ;

SCOLNICK, EM ;

SIGAL, IS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4686-4690

[6]

KRAUSSLICH HG, 1988, ANNU REV BIOCHEM, V57, P701, DOI 10.1146/annurev.bi.57.070188.003413

[7]

KRAUSSLICH HG, 1989, CURRENT COMMUNICATIO

[8]

LAPATTO P, 1989, NATURE, V342, P299

[9] CHEMICAL SYNTHESIS AND EXPRESSION OF THE HIV-1 PROTEASE GENE IN ESCHERICHIA-COLI [J].

LOUIS, JM ;

WONDRAK, EM ;

COPELAND, TD ;

SMITH, CAD ;

MORA, PT ;

OROSZLAN, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (01) :87-94

[10]

LOUIS JM, 1990, UNPUB EUR J BIOCH