ANALYSIS OF RESTRICTION ENZYME-INDUCED CHROMOSOMAL-ABERRATIONS BY FLUORESCENCE IN-SITU HYBRIDIZATION

被引:17
作者
COLUMNA, EA
GIACCIA, AJ
EVANS, JW
YATES, BL
MORGAN, WF
机构
[1] UNIV CALIF SAN FRANCISCO,RADIOBIOL & ENVIRONM HLTH LAB,BOX 0750,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,DEPT RADIAT ONCOL,SAN FRANCISCO,CA 94143
[3] STANFORD UNIV,MED CTR,SCH MED,DEPT RADIAT ONCOL,STANFORD,CA 94305
关键词
DNA DOUBLE-STRAND BREAKS; CHROMOSOME ABERRATIONS; DNA REPAIR;
D O I
10.1002/em.2850220106
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Fluorescence in situ hybridization and Giemsa staining of metaphase chromosomes were used to determine the relative frequencies of symmetric exchange aberrations (translocations) and asymmetric exchange aberrations (rings, dicentrics, and polycentrics) after exposure of human lymphoblastoid cells to restriction enzymes or X-rays. The yield of symmetric exchanges was determined with the use of chromosome-specific probes for human chromosomes 2 or 4, which were hybridized to metaphase chromosomes from cells exposed to the enzymes PvuII, SacI, or XbaI or 3 or 5 Gy of X-rays. The yield of asymmetric exchanges was determined in Giemsa-stained metaphase chromosomes from the same enzyme-treated or irradiated cell population. About 1.5- to 3-fold more symmetric than asymmetric exchanges were induced after restriction enzyme treatment. However, after X-ray treatment the yield of dicentrics relative to the yield of reciprocal translocations was close to the expected 1:1 ratio. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码:26 / 33
页数:8
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