CLONING OF THE GENE ENCODING TROP-2, A CELL-SURFACE GLYCOPROTEIN EXPRESSED BY HUMAN CARCINOMAS

被引:122
作者
FORNARO, M
DELLARCIPRETE, R
STELLA, M
BUCCI, C
NUTINI, M
CAPRI, MG
ALBERTI, S
机构
[1] IST RIC FARMACOL MARIO NEGRI, CONSORZIO MARIO NEGRI SUD, I-66030 SANTA MARIA IMBARO, ITALY
[2] UNIV NAPLES, CNR, CTR ENDOCRINOL & ONCOL SPERIMENTALE, NAPLES, ITALY
[3] UNIV NAPLES, DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC, NAPLES, ITALY
关键词
D O I
10.1002/ijc.2910620520
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have cloned by expression the cDNA encoding Trop-2, a cell-surface glycoprotein expressed by most human carcinomas. Formal proof of the identity of the clone is the hybridization to DNA and RNA from genomic TROP2 transfectants. TROP2 is a single-copy gene in human cells, hybridizes to a single 1.8-kb mRNA from expressing sources and encodes a 35,709 Da type-1 transmembrane protein with a single transmembrane domain. TROP2 is essentially identical to GA733-1. Thus, we have proven that GA733-1, for which a protein product had not been identified, is a functional gene. TROP2 is also homologous to TROP1/KSA/GA733-2, confirming the serological similarities between the 2 molecules. The homology between the Trop-1 and Trop-2 peptides is clustered in 2 extracytoplasmic domains and in the transmembrane/cytoplasmic region. Twelve cysteines and a potential cytoplasmic tyrosine phosphorylation site are also conserved. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells. On the other hand, we have found no evidence for a role of Trop-2 and Trop-1 as homophilic adhesion molecules. (C) 1995 Wiley-Liss, Inc.
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页码:610 / 618
页数:9
相关论文
共 33 条
[1]   DNA METHYLATION PREVENTS THE AMPLIFICATION OF TROP1, A TUMOR-ASSOCIATED CELL-SURFACE ANTIGEN GENE [J].
ALBERTI, S ;
NUTINI, M ;
HERZENBERG, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :5833-5837
[2]   DNA METHYLATION PREVENTS TRANSFECTION OF GENES FOR SPECIFIC SURFACE-ANTIGENS [J].
ALBERTI, S ;
HERZENBERG, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8391-8394
[3]   HIGHER TRANSFECTION EFFICIENCY OF GENOMIC DNA PURIFIED WITH A GUANIDINIUM THIOCYANATE-BASED PROCEDURE [J].
ALBERTI, S ;
FORNARO, M .
NUCLEIC ACIDS RESEARCH, 1990, 18 (02) :351-353
[4]   A SINGLE LASER METHOD FOR SUBTRACTION OF CELL AUTOFLUORESCENCE IN FLOW-CYTOMETRY [J].
ALBERTI, S ;
PARKS, DR ;
HERZENBERG, LA .
CYTOMETRY, 1987, 8 (02) :114-119
[5]   IMMUNOFLUORESCENCE ANALYSIS IN FLOW-CYTOMETRY - BETTER SELECTION OF ANTIBODY-LABELED CELLS AFTER FLUORESCENCE OVERCOMPENSATION IN THE RED CHANNEL [J].
ALBERTI, S ;
BUCCI, C ;
FORNARO, M ;
ROBOTTI, A ;
STELLA, M .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1991, 39 (05) :701-706
[6]   BIOCHEMICAL-CHARACTERIZATION OF TROP-2, A CELL-SURFACE MOLECULE EXPRESSED BY HUMAN CARCINOMAS - FORMAL PROOF THAT THE MONOCLONAL ANTIBODY-T16 AND ANTIBODY-MOV-16 RECOGNIZE TROP-2 [J].
ALBERTI, S ;
MIOTTI, S ;
STELLA, M ;
KLEIN, CE ;
FORNARO, M ;
MENARD, S ;
COLNAGHI, MI .
HYBRIDOMA, 1992, 11 (05) :539-545
[7]  
COOPER JA, 1984, J BIOL CHEM, V259, P7835
[8]  
DELEIJ L, 1994, INT J CANCER, P60
[9]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[10]   CELL-SURFACE ANTIGENS OF HUMAN BLADDER-CANCER DEFINED BY MOUSE MONOCLONAL-ANTIBODIES [J].
FRADET, Y ;
CORDONCARDO, C ;
THOMSON, T ;
DALY, ME ;
WHITMORE, WF ;
LLOYD, KO ;
MELAMED, MR ;
OLD, LJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (01) :224-228