NEW FLUOROMETRIC METHOD FOR DETERMINATION OF CORTISOL IN SERUM

被引：52

作者：

CLARK, BR

RUBIN, RT

机构：

[1] United States Navy Medical Neuropsychiatric Research Unit, San Diego

[2] Department of Psychiatry, U.C.L.A. School of Medicine, Los Angeles

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90004-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid serum cortisol method having the specificity and precision of lengthier chromatographic purification procedures is described. It uses a metaperiodate oxidation of cortisol and corticosterone to their corresponding 17β-carboxylic acids. Only the cortisol derivative is fluorescent. Forty samples may be processed in eight hours with minimal equipment and glassware. The method is easily adaptable to micro techniques. © 1969.

引用

页码：31 / &

共 27 条

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