TRIGLYCERIDE ACCUMULATION AND FATTY-ACID PROFILE CHANGES IN CHLORELLA (CHLOROPHYTA) DURING HIGH PH-INDUCED CELL-CYCLE INHIBITION

被引：149

作者：

GUCKERT, JB ^{[1
]}

COOKSEY, KE ^{[1
]}

机构：

[1] MONTANA STATE UNIV,DEPT MICROBIOL,BOZEMAN,MT 59717

来源：

JOURNAL OF PHYCOLOGY | 1990年 / 26卷 / 01期

关键词：

alkaline pH; autosporangial complexes; cell cycle inhibition; Chlorella; fatty acids; triglyceride accumulation;

D O I：

10.1111/j.0022-3646.1990.00072.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Alkaline pH stress resulted in triglyceride (TG) accumulation in Chlorella CHLOR1 and was independent of medium nitrogen or carbon levels. Based on morphological observations, alkaline pH inhibited autospore release, thus increasing the time for cell cycle completion. Autospore release has been postulated to coincide with TG utilization within the microalgal cell division cycle. The alkaline pH stress affected lipid accumulation by inhibiting the cell division cycle prior to autospore release and, therefore, prior to TG utilization. Cells inhibited in this manner showed an increase in TG accumulation but a decrease in both membrane lipid classes (glycolipid and polar lipid). Unlike TG fatty acid profiles, membrane lipid fatty acid profiles were not stable during TG accumulation. The membrane profiles became similar to the TG, i.e. less unsaturated than in the membrane lipids of unstressed control cells. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：72 / 79

页数：8

共 31 条

[1] SYNTHESIS OF CHLOROPLAST MEMBRANE LIPIDS AND CHLOROPHYLL IN SYNCHRONOUS CULTURES OF CHLAMYDOMONAS-REINHARDI [J].

BECK, JC ;

LEVINE, RP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 489 (03) :360-369

[2]

BENAMOTZ A, 1985, J PHYCOL, V21, P72

[3]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[4] OPTIMAL POLICY FOR METABOLISM OF STORAGE MATERIALS IN UNICELLULAR ALGAE [J].

COHEN, D ;

PARNAS, H .

JOURNAL OF THEORETICAL BIOLOGY, 1976, 56 (01) :1-18

[5] FLUOROMETRIC-DETERMINATION OF THE NEUTRAL LIPID-CONTENT OF MICROALGAL CELLS USING NILE RED [J].

COOKSEY, KE ;

GUCKERT, JB ;

WILLIAMS, SA ;

CALLIS, PR .

JOURNAL OF MICROBIOLOGICAL METHODS, 1987, 6 (06) :333-345

[6]

Erwin J., 1973, LIPIDS BIOMEMBRANES, P41, DOI [10.1016/B978-0-12-242050-4.50008-2, DOI 10.1016/B978-0-12-242050-4.50008-2]

[7] FATTY-ACID DYNAMICS IN THALASSIOSIRA-PSEUDONANA (BACILLARIOPHYCEAE) - IMPLICATIONS FOR PHYSIOLOGICAL ECOLOGY [J].

FISHER, NS ;

SCHWARZENBACH, RP .

JOURNAL OF PHYCOLOGY, 1978, 14 (02) :143-150

[8]

FOGG GE, 1956, ANN BOT, V10, P265

[9] BIOSYNTHESIS AND DESATURATION OF THE DIFFERENT DIACYLGLYCEROL MOIETIES IN HIGHER-PLANTS [J].

FRENTZEN, M .

JOURNAL OF PLANT PHYSIOLOGY, 1986, 124 (3-4) :193-209

[10] THE EFFECT OF CO2 ON PH IN CULTURE MEDIA FOR ALGAE [J].

GALLOWAY, RA ;

KRAUSS, RW .

PLANT AND CELL PHYSIOLOGY, 1961, 2 (03) :331-337

← 1 2 3 4 →