LYMPHOCYTE SUB-POPULATIONS IN MAN - B-CELL STIMULATION INDUCED BY POKEWEED MITOGEN AND IRRADIATED T-CELLS

被引：21

作者：

JOHNSEN, HE

MADSEN, M

机构：

[1] The Tissue Typing Laboratory, University Hospital, Aarhus

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1979年 / 10卷 / 03期

关键词：

D O I：

10.1111/j.1365-3083.1979.tb01346.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The enhanced stimulasion of human B lymphocytes by pokeweed mitogen in the presence of irradiated T helper lymphocytes has been studied, revealing that the proliferative responses measured hy incorporation of thymidine in cultures of B lymphocytes and irradiated T lymphocytes in a 1:2 or 1:4 ratio is mosily a function of the B cells. Only a minimal number, if any, of the T cells contaminating the B cell suspensions is stimulated to proliferation. This is in contrast to stimulation ofihe B‐cell suspensions without addition of irradiated T cells, where both B cells and T cells proliferate. The irradiated T helper cells have no FcR for antigen‐bound IgG, and as well allogeneic as aucologous T ceils exhibit helper capacity of equal strength. The test system described makes it possible selectively to lest one B‐cell function and the corresponding T helper capacity. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：241 / 249

页数：9

共 20 条

[1]

CHOI YS, 1977, IMMUNOLOGY, V33, P887

[2] ACTIVATION OF HUMAN B-LYMPHOCYTES .1. DIRECT PLAQUE-FORMING CELL ASSAY FOR MEASUREMENT OF POLYCLONAL ACTIVATION AND ANTIGENIC-STIMULATION OF HUMAN B-LYMPHOCYTES [J].