RUBBER TRANSFERASE-ACTIVITY IN RUBBER PARTICLES OF GUAYULE

被引：92

作者：

CORNISH, K ^{[1
]}

BACKHAUS, RA ^{[1
]}

机构：

[1] ARIZONA STATE UNIV,DEPT BOT,TEMPE,AZ 85287

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 12期

关键词：

Asteraceae; isoprenes; natural rubber biosynthesis; Parthenium argentatum; prenyl transferase; rubber particle proteins; rubber transferase; stembark;

D O I：

10.1016/0031-9422(90)85337-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rubber transferase (RuT) activity, measured as incorporation of radiolabelled isoprene from [14C]isopentenyl pyrophosphate (IPP) into rubber, was assayed in suspensions of washed rubber particles (WRPs) purified from stembark tissue of Parthenium argentatum. Isolated WRPs had high RuT activity which was not diminished even after repeated washing, demonstrating the firm association of the enzyme with the particles. The activity of RuT was characterized with respect to substrate and WRP concentration. The rate of IPP-incorporation was dependent upon the concentration of two substrates, IPP and the allylic pyrophosphate starter molecule E,E-farnesyl pyrophosphate (FPP). The enzyme present in 6 × 1010 WRPs per ml was saturated by 1 mM IPP and 20 μM FPP. Under saturating cosubstrate concentrations the apparent Km of RuT was ca 300 μM IPP and 3 μM FPP. Analysis of WRPs by SDS-PAGE revealed a simple protein profile characteristic of guayule rubber particles. A successful and facile assay for IPP-polymerization by isolated rubber particles is described. © 1990.

引用

页码：3809 / 3813

页数：5

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