ANALYSIS OF OLIGOURONATES BY REVERSED-PHASE ION-PAIR HPLC - ROLE OF THE MOBILE PHASE

被引：12

作者：

HEYRAUD, A

LEONARD, C

机构：

[1] Centre de Recherches sur les Macromolécules Végétables, CERMAV-CNRS, 38041 Grenoble Cedex

来源：

CARBOHYDRATE RESEARCH | 1991年 / 215卷 / 01期

关键词：

D O I：

10.1016/0008-6215(91)84011-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of oligouronic acids has been performed by the h.p.l.c. ion-pair method on a C18 reversed-phase column using various divalent-cation salt solutions containing 0.05% dodecyltrimethylammonium chloride. Oligogalacturonic, oligoguluronic, and oligomannuronic acids have been studied. In each series, the separation is dependent on the ionic strength of the eluent. By changing the cation, it is possible to significantly modify the resolution, and the system has been shown to be very useful, not only in the studies of selective interaction between divalent cation and oligouronates, but also in structural studies of alginates.

引用

页码：105 / 115

页数：11

共 26 条

[1] Preparation of oligoguluronides from alginate [J].