APPLICATION OF GAS-CHROMATOGRAPHY MASS-SPECTROMETRY FOR RAPID DETECTION OF MYCOBACTERIUM-XENOPI IN DRINKING-WATER

被引：19

作者：

ALUGUPALLI, S

LARSSON, L

SLOSAREK, M

JARESOVA, M

机构：

[1] UNIV LUND, DEPT MED MICROBIOL, S-22362 LUND, SWEDEN

[2] NATL INST PUB HLTH, CS-10042 PRAGUE 10, CZECHOSLOVAKIA

[3] WATERWORKS PRAGUE, CS-14700 PRAGUE, CZECHOSLOVAKIA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 11期

关键词：

D O I：

10.1128/AEM.58.11.3538-3541.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gas chromatography-mass spectrometry (GC-MS) was used to detect 2-docosanol, a secondary alcohol characteristic of Mycobacterium xenopi, in 7 of 10 analyzed drinking water samples culture positive for that species. GC-MS was also used to detect tuberculostearic acid. Both of these chemical markers were analyzed as halogenated derivatives in the negative-ion-chemical-ionization mode. The numbers of CFU of M. xenopi were lowest in the three GC-MS-negative samples. The described GC-MS method is useful for the rapid detection of M. xenopi in drinking water.

引用

页码：3538 / 3541

页数：4

共 15 条

[1] GAS-CHROMATOGRAPHY MASS-SPECTROMETRY METHODS FOR ANALYSIS OF SECONDARY ALCOHOLS PRESENT IN THE MYCOBACTERIUM-AVIUM COMPLEX [J].

ALUGUPALLI, S ;

MIELNICZUK, Z ;

LARSSON, L .

JOURNAL OF MICROBIOLOGICAL METHODS, 1992, 15 (03) :229-240

[2]

ALUGUPALLI S, UNPUB

[3]

CARSON LA, 1978, APPL ENVIRON MICROB, V36, P839, DOI 10.1128/AEM.36.6.839-846.1978

[4] MYCOBACTERIA IN WATER [J].

COLLINS, CH ;

GRANGE, JM ;

YATES, MD .

JOURNAL OF APPLIED BACTERIOLOGY, 1984, 57 (02) :193-211

[5]

du Moulin G. C., 1986, ASM NEWS, V52, P525

[6]

HORAK Z, 1986, Journal of Hygiene Epidemiology Microbiology and Immunology (Prague), V30, P405

[7]

JENKINS PA, 1991, J APPL BACTERIOL, V70, pS137

[8]

KUBIN M, 1984, MYCOBACTERIA SOURCEB, P1313

[9] ESTABLISHMENT OF 2-DOCOSANOL AS A CELLULAR MARKER COMPOUND IN THE IDENTIFICATION OF MYCOBACTERIUM-XENOPI [J].

LARSSON, L ;

JIMENEZ, J ;

VALEROGUILLEN, P ;

MARTINLUENGO, F ;

KUBIN, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (10) :2388-2390

[10] AN OUTBREAK OF MYCOBACTERIUM-TERRAE IN CLINICAL SPECIMENS ASSOCIATED WITH A HOSPITAL POTABLE WATER-SUPPLY [J].

LOCKWOOD, WW ;

FRIEDMAN, C ;

BUS, N ;

PIERSON, C ;

GAYNES, R .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1989, 140 (06) :1614-1617

← 1 2 →