PROGESTERONE INCREASES CA2+ CURRENTS IN MYOMETRIAL CELLS FROM IMMATURE AND NONPREGNANT ADULT-RATS

被引：37

作者：

RENDT, JM

TORO, L

STEFANI, E

ERULKAR, SD

机构：

[1] UNIV PENN,SCH MED,DEPT PHARMACOL,PHILADELPHIA,PA 19104

[2] BAYLOR COLL MED,DEPT MOLEC PHYSIOL & BIOPHYS,HOUSTON,TX 77030

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 02期

关键词：

OVARIAN STEROIDS; WHOLE CELL VOLTAGE CLAMP; CALCIUM CHANNELS;

D O I：

10.1152/ajpcell.1992.262.2.C293

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We examined the role of ovarian steroids in regulating Ca2+ channels in rat uterine smooth muscle. Ca2+ currents (I(Ca)) in myometrial cells from nonpregnant adult rats and immature rats injected with either estrogen or progesterone or estrogen plus progesterone were measured with the whole cell patch-clamp method. I(Ca) was more prominent in cells from diestrous rats than in cells from estrous rats. In cells from immature rats the I(Ca) density was significantly greater in cells from progesterone-injected rats than in cells from estrogen-injected or noninjected rats. I(Ca) in cells from rats injected simultaneously with progesterone and the progesterone antagonist RU-486 was not significantly greater than those from noninjected rats. These increases in I(Ca) density are not the result of changes in I(Ca) activation kinetics or voltage dependence, since both are unaffected by steroid injection. The kinetics and voltage dependence of the I(Ca) current in cells from immature and nonpregnant adult rats are similar, suggesting that they represent a single population of Ca2+ channels.

引用

页码：C293 / C301

页数：9

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