IS THE VERTICAL DISPOSITION OF MYCOPLASMA MEMBRANE-PROTEINS AFFECTED BY MEMBRANE FLUIDITY

被引：29

作者：

AMAR, A

ROTTEM, S

RAZIN, S

机构：

[1] Biomembrane Research Laboratory, Department of Clinical Microbiology, The Hebrew University-Hadassah Medical School, Jerusalem

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 552卷 / 03期

关键词：

(Acholeplasma laidlawii); Lactoperoxidase-mediated iodination; Membrane fluidity; Vertical disposition;

D O I：

10.1016/0005-2736(79)90190-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the physical state of the membrane lipid matrix on the vertical disposition of membrane proteins was studied with Acholeplasma laidlawii. Changes in membrane fluidity were brought about by altering the fatty acid composition of membrane lipids, by changing the growth temperature, by aging of cultures and by inducing changes in the membrane lipid-to-protein ratio through treatment with chloramphenicol. The lactoperoxidase-mediated iodination technique was used to label membrane proteins exposed to the aqueous surroundings. The degree of exposure of the iodine-binding sites of membrane proteins on the external surface of intact cells was found to undergo significant changes on varying growth conditions, but the changes could not be consistently correlated with changes in membrane fluidity, nor were they discernible on iodination of isolated membranes. © 1979.

引用

页码：457 / 467

页数：11

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