OVEREXPRESSION, PURIFICATION AND CRYSTALLIZATION OF BAMHI ENDONUCLEASE

被引：29

作者：

JACK, WE

GREENOUGH, L

DORNER, LF

XU, SY

STRZELECKA, T

AGGARWAL, AK

SCHILDKRAUT, I

机构：

[1] NEW ENGLAND BIOLABS INC,32 TOZER RD,BEVERLY,MA 01915

[2] COLUMBIA UNIV COLL PHYS & SURG,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 08期

关键词：

D O I：

10.1093/nar/19.8.1825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 angstrom in X-ray analysis.

引用

页码：1825 / 1829

页数：5

共 27 条

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