SOLID-PHASE SYNTHESIS OF DIRECTLY LINKED PEPTIDE-OLIGODEOXYNUCLEOTIDE HYBRIDS USING STANDARD SYNTHESIS PROTOCOLS

被引：49

作者：

BERGMANN, F

BANNWARTH, W

机构：

[1] HOFFMANN LA ROCHE INC,ANTISENSE RES,NUTLEY,NJ 07110

[2] F HOFFMANN LA ROCHE & CO LTD,PRPC,CH-4002 BASEL,SWITZERLAND

来源：

TETRAHEDRON LETTERS | 1995年 / 36卷 / 11期

关键词：

D O I：

10.1016/0040-4039(95)00155-6

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We describe a general methodology for the solid phase synthesis of directly linked peptide-oligonucleotide hybrids using a sarcosine-modified controlled pore glass (CPG) support and employing phosphoramidite chemistry for the oligonucleotide synthesis and Fmoc strategy for the peptide assembly. Using this approach a special linker is not needed and the resultant peptide-oligonucleotide hybrid can be further elongated with an oligonucleotide sequence.

引用

页码：1839 / 1842

页数：4

共 27 条

[1] BANNWARTH W, 1987, CHIMIA, V41, P302
[2] SOLID-PHASE SYNTHESIS OF OLIGODEOXYNUCLEOTIDES CONTAINING PHOSPHORAMIDATE INTERNUCLEOTIDE LINKAGES AND THEIR SPECIFIC CHEMICAL CLEAVAGE
BANNWARTH, W
[J]. HELVETICA CHIMICA ACTA, 1988, 71 (06) : 1517 - 1527
[3] BERGMANN F, UNPUB TETRAHEDRON
[4] ENCODED COMBINATORIAL CHEMISTRY
BRENNER, S
LERNER, RA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) : 5381 - 5383
[5] A NEW BASE-STABLE LINKER FOR SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS
BROWN, T
PRITCHARD, CE
TURNER, G
SALISBURY, SA
[J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1989, (14) : 891 - 893
[6] 1-HYDROXY-7-AZABENZOTRIAZOLE - AN EFFICIENT PEPTIDE COUPLING ADDITIVE
CARPINO, LA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (10) : 4397 - 4398
[7] STEPWISE SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDE-PEPTIDE HYBRIDS
DELATORRE, BG
AVINO, A
TARRASON, G
PIULATS, J
ALBERICIO, F
ERITJA, R
[J]. TETRAHEDRON LETTERS, 1994, 35 (17) : 2733 - 2736
[8] SOLID-PHASE SYNTHESIS OF THE NUCLEOPEPTIDE FRAGMENT H-ASP-SER[PAAAGTAAGCC]-GLU-OH FROM THE NUCLEOPROTEIN OF BACILLUS-SUBTILIS PHAGE PHI-29
DREEFTROMP, CM
VANDERMAAREL, JCM
VANDENELST, H
VANDERMAREL, GA
VANBOOM, JH
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (15) : 4015 - 4020
[9] SOLID-PHASE SYNTHESIS OF H-PHE-TYR-(PATAT)-NH2 - A NUCLEOPEPTIDE FRAGMENT FROM THE NUCLEOPROTEIN OF BACTERIOPHAGE-PHI-X174
DREEFTROMP, CM
VANDAM, EMA
VANDENELST, H
VANDERMAREL, GA
VANBOOM, JH
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (22) : 6491 - 6495
[10] SYNTHESIS OF DEFINED PEPTIDE-OLIGONUCLEOTIDE HYBRIDS CONTAINING A NUCLEAR TRANSPORT SIGNAL SEQUENCE
ERITJA, R
PONS, A
ESCARCELLER, M
GIRALT, E
ALBERICIO, F
[J]. TETRAHEDRON, 1991, 47 (24) : 4113 - 4120

← 1 2 3 →