USE OF NONDENATURING SILVER-STAINED POLYACRYLAMIDE-GEL ANALYSIS OF POLYMERASE CHAIN-REACTION AMPLIFICATION PRODUCTS FOR THE DIFFERENTIAL-DIAGNOSIS OF LEPTOSPIRA-INTERROGANS INFECTION

被引：9

作者：

OLIVEIRA, MAA

CABALLERO, OL

NETO, ED

KOURY, MC

ROMANHA, AJ

CARVALHO, J

HARTSKEERL, RA

SIMPSON, AJG

机构：

[1] LUDWIG INST CANC RES,BR-01509010 SAO PAULO,BRAZIL

[2] FIOCRUZ MS,CTR PESQUISAS RENE RACHOU,BELO HORIZONT,MG,BRAZIL

[3] UNIV FED MINAS GERAIS,DEPT MICROBIOL,BELO HORIZONT,MG,BRAZIL

[4] FUNED,FUNDACAO EZEQUIEL DIAS,BELO HORIZONT,MG,BRAZIL

[5] FAC MED TRIANGULO MINEIRO,DEPT CIENCIAS BIOL,UBERABA,MG,BRAZIL

[6] UNIV FED MINAS GERAIS,DEPT BIOQUIM & IMUNOL,BELO HORIZONT,MG,BRAZIL

[7] ROYAL TROP INST,DEPT BIOMED RES,1105 AZ AMSTERDAM,NETHERLANDS

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1995年 / 22卷 / 04期

关键词：

D O I：

10.1016/0732-8893(95)00145-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A 285-bp DNA fragment was amplified using the polymerase chain reaction from 38 Leptospira serovars of six different genomic species. The fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. Leptospira interrogans serovars could be distinguished from those of other species on this basis.

引用

页码：343 / 348

页数：6

共 14 条

[1] PENICILLINS, CEPHALOSPORINS, AND TETRACYCLINES IN TREATMENT OF HAMSTERS WITH FATAL LEPTOSPIROSIS [J].