A TRIGGERING STRUCTURE RECOGNIZED BY G7 MONOCLONAL-ANTIBODY ON PORCINE LYMPHOCYTES AND GRANULOCYTES

被引:36
作者
DATO, ME [1 ]
WIERDA, WG [1 ]
KIM, YB [1 ]
机构
[1] UNIV HLTH SCI CHICAGO MED SCH, DEPT MICROBIOL & IMMUNOL, 3333 GREEN BAY RD, N CHICAGO, IL 60064 USA
关键词
D O I
10.1016/0008-8749(92)90212-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and >95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of ~40 kDa. Functionally, whole but not F(ab′)2 fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab′)2 fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity. © 1992.
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页码:468 / 477
页数:10
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