PREPARATION OF FINE MAGNETIC PARTICLES AND APPLICATION FOR ENZYME IMMOBILIZATION

被引：79

作者：

SHINKAI, M ^{[1
]}

HONDA, H ^{[1
]}

KOBAYASHI, T ^{[1
]}

机构：

[1] NAGOYA UNIV, FAC ENGN, DEPT CHEM ENGN, CHIKUSA KU, NAGOYA 46401, JAPAN

来源：

BIOCATALYSIS | 1991年 / 5卷 / 01期

关键词：

FERROFLUID; MAGNETIC PARTICLES; AMINOSILANE; GLUTARALDEHYDE; ENZYME IMMOBILIZATION; COVALENT BINDING;

D O I：

10.3109/10242429109014855

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fine magnetic particles (ferrofluid) were prepared from a co-precipitation method by oxidation of Fe2+ with nitrite. The particles were activated with (3-aminopropyl)triethoxysilane in toluene and the activated particles were combined with some enzymes by using glutaraldehyde. Enzyme-immobilized magnetic particles were between 4-70 nm and the size could be changed corresponding to the ratio of the amount of Fe2+ to that of nitrite. In the immobilization of βglucosidase, activity yield was 83% and 168 mg protein was immobilized per g magnetite. Other enzymes or proteins could be immobilized at the level between about 70 and 200mg/g support. Immobilized βglucosidase was stable at 4°C. Magnetic particles immobilized with βglucosidase responded quickly to the magnetic field and "ON-OFF" control of the enzyme reaction was possible. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：61 / 69

页数：9

共 12 条

[1] CELLULASE IMMOBILIZATION ON FE3O4 AND CHARACTERIZATION [J].

GARCIA, A ;

OH, S ;

ENGLER, CR .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (03) :321-326

[2] ALBUMIN MICROSPHERES .3. SYNTHESIS AND CHARACTERIZATION OF MICROSPHERES CONTAINING ADRIAMYCIN AND MAGNETITE [J].

GUPTA, PK ;

HUNG, CT ;

LAM, FC ;

PERRIER, DG .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1988, 43 (1-2) :167-177

[3] MOLECULAR-CLONING AND EXPRESSION OF A BETA-GLUCOSIDASE GENE FROM RUMINOCOCCUS-ALBUS IN ESCHERICHIA-COLI [J].

HONDA, H ;

SAITO, T ;

IIJIMA, S ;

KOBAYASHI, T .

ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (09) :559-562

[4] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODY TO RECOMBINANT ALPHA-AMYLASE [J].

KAMIHIRA, M ;

KAWAKUBO, I ;

TANIGUCHI, M ;

IIJIMA, S ;

KOBAYASHI, T .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1988, 21 (04) :357-362

[5]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[6]

MATSUNAGA T, 1987, APPL MICROBIOL BIOT, V26, P328

[7] STABILITY OF A RECOMBINANT PLASMID CONTAINING ALPHA-AMYLASE GENE IN CHEMOSTAT [J].

MIZUTANI, S ;

FUKUZONO, S ;

TSUKAGOSHI, N ;

UDAKA, S ;

KOBAYASHI, T .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1985, 18 (03) :220-224

[8] IMMUNOSPECIFIC FERROMAGNETIC IRON-DEXTRAN REAGENTS FOR THE LABELING AND MAGNETIC SEPARATION OF CELLS [J].

MOLDAY, RS ;

MACKENZIE, D .

JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 52 (03) :353-367

[9]

REIMERS GM, 1976, Patent No. 1439031

[10] ENHANCEMENT OF OXYGEN ABSORPTION BY MAGNETITE-CONTAINING BEADS OF IMMOBILIZED GLUCOSE-OXIDASE [J].

SADA, E ;

KATOH, S ;

TERASHIMA, M .

BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (05) :1037-1044

← 1 2 →