FORMATION OF INOSINE TRIPHOSPHATE AND OF 14C-LABELED 2,6-DIAMINOPURINE RIBONUCLEOSIDE DI- AND TRIPHOSPHATES IN STORED HUMAN ERYTHROCYTES

Inosine triphosphate (ITP) and 14C‐labeled 2,6‐diaminopurine ribonucleoside di‐ and triphosphate (DDP‐14C and DTP‐14C, respectively), were formed during incubation of 2,6‐diaminopurine‐2‐14C and inosine with stored human erythrocytes at 37 C for five hours. Erythrocytes were obtained from acid‐citrate‐dextrose blood which had been stored in a blood bank for four weeks. DDP‐14C, DTP‐14C, and ITP were isolated and purified by chromatography on Dowex‐1 formate resin and on paper, and identified by their chromatographic mobilities, fluorescence in ultraviolet light, derivatives, and absorption spectra. The yields of DDP‐14C, DTP‐14C, and ITP were 0.16, 1.13, and 0.07 per cent, respectively, of the added 2,6‐diaminopurine‐2‐14C or inosine. 1969 AABB