ON MECHANISM OF ENZYMATIC CONVERSION OF CHOLEST-5ENE-3BETA,7ALPHA-DIOL INTO 7ALPHA-HYDROXYCHOLEST-4-EN-3-ONE

被引：62

作者：

BJORKHEM, I

机构：

[1] Kemiska Institutionen, Karolinska Institutet, Stockholm, S-10401

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 8卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00533.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conversion of cholest‐5‐ene‐3β,7α‐diol into 7α‐hydroxycholest‐4‐en‐3‐one in the presence of rat liver microsomes fortified with NAD was studied with the aid of [4‐14C]‐, [3α‐3H]‐, [4β‐3H]‐, and [4β‐2H]cholest‐5‐ene‐3β,7α‐diol. The reaction was also carried out in 3H2O and 2H2O and the incorporation of isotope in the product was determined. It was found that the rate‐limiting step in the reaction involved loss of the 3α‐hydrogen. This step was coupled with or followed by loss of hydrogen from the 4β‐position, of which a small amount was transferred to the 6β‐position of the product. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：337 / &

共 33 条

[1] BILE ACIDS AND STEROIDS .18. STUDIES ON THE 7ALPHA-HYDROXYLATION OF TAURODESOXYCHOLIC ACID IN RAT LIVER HOMOGENATES [J].